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Is Your Gut Leaking Find Out with a Home Test

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Published By: Dr. Jeffrey Mark
Date: December 28, 2023


“Leaky gut” or intestinal permeability occurs when normal single layer protective barrier of the GI tract is breached by incompletely digested food or bacteria. This has been correlated with an elevation of a protein called Zonulin which can be measured. Other markers of damage include anitbodies to Vinculin and antibodies to cytolethal distending toxin B (CdtB). Leaky gut can lead to food sensitivities, autoimmune conditions, and anxiety among other conditions.  Fortunately you can repair the lining through nutraceuticals, diet, and lifestyle changes as discussed in this article.


Embarking on a journey to understand and optimize your gut health is a crucial step towards overall well-being. The digestive system, often referred to as the body's "second brain," plays a pivotal role in maintaining health.

Recently, the concept of "leaky gut syndrome" has gained prominence, signaling the potential impact of a compromised intestinal barrier on various aspects of health.

Leaky gut, scientifically known as increased intestinal permeability, refers to a condition where the lining of the intestines becomes more porous than normal. This heightened permeability allows substances, such as undigested food particles and bacteria, to pass through the intestinal wall and enter the bloodstream.

The body's immune system may then react to these invaders, potentially leading to inflammation and a range of health issues.

In this comprehensive guide, we will explore the signs and symptoms of leaky gut syndrome, discuss available at-home testing methods, and provide insights into lifestyle modifications that can support a healthier gut.

By the end, you'll be equipped with the knowledge to assess and enhance your gut health from the comfort of your own home.

Join us on this journey to unravel the mysteries of your gut and empower yourself with the tools to make informed decisions for a healthier, more resilient you. Let's dive into the intricate world of leaky gut syndrome and explore how you can take control of your digestive wellness.

Understanding Leaky Gut

Understanding Leaky Gut

To comprehend leaky gut syndrome, it's essential to grasp the intricacies of the intestinal barrier. The intestines, specifically the small intestine, act as a selective barrier designed to absorb nutrients while preventing harmful substances from entering the bloodstream.

There is a single layer of cells lined up side by side like a picket fence. Substances can travel through each cell, through the “planks” in between cells or in between the “planks.”

Under normal circumstances, the cells lining the intestinal wall, the “picket fence” are closely packed together, forming a tight barrier.

However, when this barrier becomes compromised, as is the case with leaky gut syndrome, the gaps between the cells widen, allowing unwanted particles to pass through into the bloodstream.

This breach triggers an immune response, potentially leading to inflammation and a cascade of health issues.

Signs and Symptoms of Leaky Gut

Recognizing the signs of a leaky gut is the first step toward understanding your gut health. While symptoms can vary, common indicators include:

  1. Digestive Issues: Persistent gas, diarrhea, constipation, or irritable bowel syndrome (IBS) symptoms may signal digestive distress. If you are wondering Why Am I So Bloated and Gassy, it is one of the symptoms of a leaky gut.
    Digestive issues
  2. Food Sensitivities: Developing sensitivities or allergies to certain foods, especially those previously well-tolerated.
  3. Autoimmune Conditions: Some individuals with leaky gut may experience an increased risk of autoimmune diseases, where the immune system mistakenly attacks the body's own tissues.
  4. Nonspecific General Symptoms: Headache/ migraines, brain fog, mood instabilities like depression/anxiety, ADD/ ADHD, fatigue/ low energy, arthritis, nutrient deficiencies
  5. Joint Pain and Muscle Aches: Inflammation triggered by a leaky gut can manifest as joint pain and generalized muscle discomfort.
    Joint Pain and Muscle Aches
  6. Skin Conditions: Conditions like eczema or psoriasis may be linked to gut health, with leaky gut potentially contributing to skin inflammation.

Other Associated Disorders with Leaky Gut

  1. Inflammatory Bowel Disease, ulcerative colitis/ Crohn’s disease
  2. Rheumatoid arthritis
  3. Fibromyalgia

Understanding these potential indicators is crucial for early detection and intervention.

Home-Based Testing for Leaky Gut

While definitive diagnosis often requires professional medical assessment, there are several at-home methods to assess the possibility of leaky gut but these tests that measure markers such as Zonulin, anti-vinculin, and anti-cytolethal distending toxin B (CdtB) may not correlate 100% with leaky gut.

Conventional lab tests will also often back as all “within normal limits.” These tests are not designed to measure optimal function or necessarily detect leaky gut.  Tests that may correlate with leaky gut include:

  1. Food sensitivity testing. These tests can determine if an underlying food sensitivity or intolerance is driving inflammation in the gut. Repeated testing is often used after treatment trials.
    1. How They Work: There are at-home tests that utilize a specialized paper to collect dried blood spots. These kits typically involve collecting a small blood sample at home, which is then sent to a laboratory for analysis. The results indicate your body's immune response to various foods
    2. A less precise alternative may be an elimination diet to eliminate gluten, sugar, eggs, milk, dairy, peanuts, and shellfish for 6 weeks then reintroducing them back 1 at a time every 3 days.
    3. Interpreting Results: Identifying foods that provoke an immune response can guide personalized dietary changes. However, it's essential to approach these results with caution and consult with a healthcare professional for interpretation.
      1. High Reactivity: Foods that elicit a strong immune response may be categorized as high-reactivity. Consider eliminating or reducing these foods from your diet for a trial period.
      2. Moderate Reactivity: Moderate-reactivity foods may be reintroduced cautiously, and their impact on symptoms monitored. Gradual reintroduction can help identify specific triggers.
  • Low Reactivity: Foods with low reactivity are generally well-tolerated. However, individual responses can vary, and it's essential to consider these results in the context of your overall health.
  1. Microbiome stool testing with Zonulin. Higher levels of Zonulin have correlated with an increased risk of intestinal permeability. Sometimes the Zonulin level may not be significantly elevated but there may be an imbalance of gut bacteria a parasite, or an overgrowth of bacteria all of which can result in a leaky gut.
    1. Collecting a Sample: At-home stool sample kits often include instructions for collecting a small sample. This sample is then sent to a laboratory for analysis.
    2. Insights into Gut Microbiome: Stool tests can provide information about the diversity and composition of your gut microbiome, offering insights into potential imbalances.
    3. While at-home stool sample analysis offers valuable insights, interpreting the results may require professional guidance. Consult with a healthcare provider to understand the implications of your results.
  2. Four-point cortisol and other hormone testing. High cortisol and altered cortisol levels may lead to shunting of blood away from the intestines leading to damage to the intestinal cell walls and leaky gut.
  3. Micronutrient testing. Nutrients, especially vitamin D, may be low and affect the healing and repair of the tissues including the intestines.
  4. Toxin testing. Evaluating for high heavy metal levels or mold may provide other treatable cases of inflammation leading to leaky gut.

How to Heal Leaky Gut

Heal Leaky Gut

While at-home testing methods provide valuable information, addressing leaky gut often requires an integrative approach. Oftentimes, because testing may not be 100% correlated with the symptoms and results of a leaky gut, empiric treatment is a reasonable alternative option.

Here is an overview of a 5 step process to heal and restore a healthy gut lining leading to good gut health that can be implemented by a functional medicine practitioner.

  1. Remove exposure or consumption of any known sensitive/intolerant foods, environmental and chronic stress, and toxins. The previously mentioned tests for food sensitivity heavy metal, mold, and hormone testing may help guide the removal process.  A 6 food elimination diet removing gluten soy, corn, dairy, milk, peanuts, and shellfish may be used as a trial in the absence of testing.
  2. Replace nutrients and enzymes based on lab results.
  3. Re-inoculate: A functional medicine approach to rebuild or support a healthy gut microbiome. Diets full of prebiotics, fiber, and fermented foods help to support a healthy gut microbiome. Nutraceuticals, supplements, and probiotics are also utilized.
  4. Repair: Repairing damage to the gut lining takes time and doesn’t happen overnight. In the short term, supplementation can support the healing process. This is achieved in the long term by removing toxins and inflammatory foods and providing proper nutrients to the body through a healthy diet and lifestyle.
  5. Rebalance the body. This includes mind, body, sleep, and nutrition. Ultimately, this is going to heal the gut for good and prevent a leaky gut from occurring again in the future.

Here are some lifestyle modifications to support a healthier gut:

  1. Balanced Diet: Prioritize a diet rich in fiber, fruits, vegetables, and lean proteins. These foods promote a diverse and healthy gut microbiome.
    balanced diet
  2. Probiotics and Prebiotics: Incorporate probiotic-rich foods, such as yogurt and fermented vegetables, to support the growth of beneficial bacteria. Prebiotics, found in foods like garlic and onions, provide nourishment for these bacteria.
  3. Hydration: Drink an adequate amount of water to support digestion and maintain overall gut health.
  4. Stress Management: Chronic stress can contribute to gut issues. Explore stress-reducing practices such as meditation, yoga, or deep breathing exercises.
    Manage Stress
  5. Regular Exercise: Physical activity promotes a healthy gut and can contribute to overall well-being.
    Exercises regularly

Here are some suggestions for supplementation to heal leaky gut:

  1. Nutrients
    1. Vitamins A & D are essential for proper gut function. Checking these vitamin levels and making sure they are optimized can help reduce inflammation.
    2. Zinc is essential for overall wound healing in the body.
    3. The amino acids Glutamine and Arginine have been studied, and supplementation has been supported to be anti-inflammatory and help heal the gut lining.
    4. Potent antioxidant and anti-inflammatory supplements like omega-3 fatty acids and curcumin reduce inflammation in the body and the gut.
    5. Butyrate has been shown to help improve gut cell synthesis, and Inulin has also been shown to help heal the gut lining.
  2. Fiber/ Prebiotics/ Probiotics
    1. Support a healthy and diverse gut microbiome
    2. Examples include root vegetables, green bananas, and cold-baked potatoes.
  3. Digestive enzymes. Enzymes such as amylase, lipase, protease, and betaine hydrochloride help complete the steps of digestion and minimize incompletely digested nutrients to inflame the immune system.

Conclusion: Taking Charge of Your Gut Health

In conclusion, your journey to better gut health involves a combination of self-awareness, at-home testing, and proactive lifestyle changes. Armed with knowledge from food sensitivity testing and stool sample analysis, you may have the tools to make informed decisions about your diet and overall well-being.

While it may seem overwhelming or confusing to figure out what may be causing your leaky gut symptoms, you don’t have to go it alone, and you don’t have to guess. By working with an Integrative or Functional Medicine health practitioner, you can determine the underlying cause of your leaky gut and develop a personalized treatment plan to resolve the symptoms.

Remember, gut health is dynamic, and the key is to adopt sustainable lifestyle changes once your leaky gut is restored. By taking charge of your gut health, you're investing in a foundation for overall wellness and vitality.

Take Care Stay Healthy,

Jeffrey Mark, M.D.

The Real Gut Doctor

Additional Resources for Further Reading

Your journey to better gut health doesn't end here. Explore the following resources and articles to deepen your understanding, discover new insights, and stay informed about the latest developments in digestive wellness:

  1. American Gastroenterological Association (AGA):
  • The AGA provides comprehensive resources on digestive health, including information on various gastrointestinal conditions and treatment options.
  1. International Probiotics Association:
  • Stay updated on the latest research and news related to probiotics. The International Probiotics Association offers a wealth of information on the science behind these beneficial microorganisms.
  1. Functional Medicine Institute:
  • Explore the principles of functional medicine, which focuses on addressing the root causes of health issues. This approach often considers the interconnectedness of various bodily systems, including the gut.


The information provided in this guide is for educational and informational purposes only and should not be considered medical advice. It is not intended to replace consultation with a qualified healthcare professional. Always seek the advice of your physician or another qualified health provider with any questions you may have regarding a medical condition.

While efforts have been made to ensure the accuracy of the information presented, developments in medical research may lead to updates and changes. The author and publisher make no representations or warranties of any kind, express or implied, about the completeness, accuracy, reliability, suitability, or availability with respect to the information, products, services, or related graphics contained in this guide.

Any reliance you place on the information provided is strictly at your own risk. The author and publisher disclaim any responsibility for any liability, loss, or risk incurred as a consequence, directly or indirectly, of the use and application of any information presented in this guide.

It is essential to consult with a qualified healthcare professional for individualized advice and guidance tailored to your specific health needs. If you are experiencing a medical emergency, call your doctor or emergency services immediately.

By reading this guide, you acknowledge and agree to the terms of this disclaimer. Your health is a personal matter, and decisions regarding your well-being should be made in consultation with a healthcare professional.


  1. Fasano, A. (2012). Leaky gut and autoimmune diseases. Clinical Reviews in Allergy & Immunology, 42(1), 71-78.
  2. Mu, Q., Kirby, J., Reilly, C. M., & Luo, X. M. (2017). Leaky gut as a danger signal for autoimmune diseases. Frontiers in Immunology, 8, 598.
  3. Hollon, J., Puppa, E. L., Greenwald, B., & Goldberg, E. (2015). Effect of gliadin on permeability of intestinal biopsy explants from celiac disease patients and patients with non-celiac gluten sensitivity. Nutrients, 7(3), 1565-1576.
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Dr. Jeffrey Mark
With over thirty years of experience, Dr. Mark is a leading expert in holistic gut health. His integrative approach combines conventional medicine, functional and regenerative medicine, and advanced therapies to heal the gut and transform patient health. Holding 5 board certifications, Dr. Mark offers the comprehensive expertise of five medical specialists during each patient visit. He is dedicated to optimizing wellbeing by addressing the gut-related root causes of chronic health issues.
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